Journal: Immunity

Article Title: The HVEM-BTLA Axis Restrains T Cell Help to Germinal Center B Cells and Functions as a Cell-Extrinsic Suppressor in Lymphomagenesis

doi: 10.1016/j.immuni.2019.05.022

Figure Lengend Snippet: BTLA on T Cells Restrains GC B Cell Competitiveness (A) Flow-cytometric analysis of BTLA expression on B cells (left) and CD4 + T cells (right) compared with Btla −/− cells. (B) HVEM-overexpressing Btla −/− BM chimeras were made by transduction of empty vector (EV) or HVEM (WT) MSCV-Thy1.1 constructs into Btla −/− BM and reconstitution of CD4-depleted hosts. Chimeras were then immunized with SRBCs, and the participation of Thy1.1 + cells in GC versus Fo B cells was determined. (C) Contribution of Btla +/+ or Btla −/− CD45.2 cells to Fo and GC populations in the spleen of mixed BM chimeras made with ∼40% CD45.2 and ∼60% WT CD45.1 at day 7 after SRBC immunization. (D) Experimental scheme and timeline for experiments in (E) and (F). (E) Ratio of the frequency of CD45.1/2 Hvem −/− HEL + B cells in BTLA-B-cell-deficient ( Btla f/f Mb1 Cre ) hosts at day 5 to the pre-transfer frequency. (F) Ratio of CD45.1/2 Hvem +/+ or Hvem −/− HEL + GC B cells to the pre-transfer mix in BTLA-T-cell-deficient ( Btla f/f Cd4 Cre ) hosts at days 3–6. Data are pooled from four experiments. Unpaired two-tailed Student’s t test (B, C, E, and F): ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ Figure S3 .

Article Snippet: CD4 PE-CY7 (clone GK1.5) , BD Bioscience , #552775.

Techniques: Expressing, Transduction, Plasmid Preparation, Construct, Two Tailed Test

Journal: Immunity

Article Title: The HVEM-BTLA Axis Restrains T Cell Help to Germinal Center B Cells and Functions as a Cell-Extrinsic Suppressor in Lymphomagenesis

doi: 10.1016/j.immuni.2019.05.022

Figure Lengend Snippet:

Article Snippet: CD4 PE-CY7 (clone GK1.5) , BD Bioscience , #552775.

Techniques: Recombinant, Flow Cytometry, Isolation, RNA Sequencing Assay, Derivative Assay, Plasmid Preparation, Software